Library of the Biotechnology Department

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Preface . . v
Contributors . . ix

1 Bioluminescent Assays for Cytochrome P450 Enzymes . . 1
2 Simultaneous Determination of Multiple GYP Inhibition Constants using a Cocktail-Probe Approach . . 11
3 High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening . . 25
4 The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites . . 51
5 High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators . . 61
6 Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches . . 71
7 Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis . . 79
8 An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins . . 95
9 Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group . . 107
10 Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes . . 115
11 Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles . . 129
12 Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells . . 135
13 Screening for Cytochrome P450 Reactivity with a Reporter Enzyme . . 149
14 High-Throughput Fluorescence Assay of Cytochrome P450 3A4 . . 157
15 Targeted Protein Capture for Analysis of Electrophile-Protem Adducts . . 163
16 DNA Shuffling of Cytochrome P450 Enzymes . . 177
17 Measurement of P450 Difference Spectra Using Intact Cells . . 189
18 DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production . . 205
19 P450 Oxidoreductase: Genotyping, Expression, Purification of Recombinant Protein, and Activity Assessments of Wild-Type and Mutant Protein . . 225
20 LICKED: A Versatile Drop-In Vector for Rapid Generation of Redox-Sclf-Sufficient Cytochromcs P450 . . 239
21 Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-Allele) Nomenclature Database . . 251
22 Simultaneous In Vivo Phenotyping of CYP Enzymes . . 261
23 Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase I Footprinting . . 269
24 Isolation of Mouse Hepatocytes . . 283
25 Highly Efficient SiRNA and Gene Transfer into Hepatocyte-Like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies . . 295

Index . . 315

Preface . . xxiii

Part 1 - Research Biographies . . 1

Chapter 1 Cristina Andres-Lacueva . . 3
Chapter 2 Gry Aletta Bjorlykke . . 9
Chapter 3 Victorio Cadierno . . 11
Chapter 4 Wisit Cheungpasitporn . . 17
Chapter 5 Marcio Jose da Silva . . 21
Chapter 6 Isabella Denis . . 25
Chapter 7 Mark D. Distefano . . 27
Chapter 8 Azza All Kamel El-Sheikh . . 39
Chapter 9 Boris Estrine . . 45
Chapter 10 Tulio Cesar Ferreira . . 47
Chapter 11 Rose Gasnier . . 49
Chapter 12 Suresh Kumar Kailasa . . 51
Chapter 13 Jung-Bum Lee . . 53
Chapter 14 C.-Y. Cynthia Lin . . 57
Chapter 15 Sigrid Mennickent . . 63
Chapter 16 Igho Onakpoya . . 67
Chapter 17 Hugo H. Ortega . . 69
Chapter 18 Paolo Polidori . . 77
Chapter 19 Marzia Salmaso . . 81
Chapter 20 Luas Adriano Santos Do Nascimento . . 83
Chapter 21 Elena Timofeeva . . 85
Chapter 22 Ana Villares . . 87
Chapter 23 Vesna Vucic . . 89
Chapter 24 Paulus S. Wang . . 91
Chapter 25 William R. Ware . . 97
Chapter 26 Anna Watson . . 99
Chapter 27 Lei Xu . . 103
Chapter 28 Oguzhan Yildiz . . 105

Part 2 - Research Summaries on Biochemistry . . 109

Chapter 29 57Fe Mossbauer Spectroscopic and Density Functional Theory (DFT) Study on the Interactions of the Metal Ion with Monosaccharides . . 111
Chapter 30 An Introductory Course on Group Theory and Chemical Applications . . 113
Chapter 31 Aromatic Polyethers Based on Heterocyclic Monomers . . 115
Chapter 32 Bacteriophages and Biofilms: Ecology, Phage Therapy, Plaques . . 117
Chapter 33 Beta-Lactamases . . 119
Chapter 34 Biflavonoids: Occurrence, Structural Features and Bioactivity . . 121
Chapter 35 Binary Aqueous and CO2 Containing Mixtures and the Krichevskii Parameter . . 123
Chapter 36 Bioactive Oligosaccharides: Production, Biological Functions and Potential Commercial Applications . . 125
Chapter 37 Biochemistry and Function of Antifreeze Proteins . . 127
Chapter 38 Biochemistry of Prenatal Alcohol Exposure . . 129
Chapter 39 Biodegradation of Cellulose Fibers . . 131
Chapter 40 Biogenic Amines: Pharmacological, Neurochemical and Molecular Aspects in the CNS . . 133
Chapter 41 Biophysics of DNA-Antibiotic Complexes . . 135
Chapter 42 Borates: Chemical, Pharmaceutical and Pharmacological Aspects . . 137
Chapter 43 Calixarene Complexes with Solvent Molecules . . 139
Chapter 44 Chemical and Biochemical Kinetics: New Perspectives . . 141
Chapter 45 Chemical and Biochemical Reactions: Kinetics and Mechanism . . 143
Chapter 46 Chemical Reactions in Gas, Liquid and Solid Phases: Synthesis, Properties and Application . . 145
Chapter 47 Chemicals and Radioactive Materials and Human Development . . 147
Chapter 48 Chitosan-Based Hydrogels for Tissue Engineering Applications . . 149
Chapter 49 Computational Metabolomics . . 151
Chapter 50 Copolymers in the Preparation of Parenteral Drug Delivery Systems . . 153
Chapter 51 Covalently and Noncovalently Bound Assemblies of Calixarenes . . 155
Chapter 52 Current Status of Chitosan on Dermal/Transdermal Drug Delivery Systems . . 157
Chapter 53 Decreasing Oxidative Stress and Retarding the Aging Process . . 159
Chapter 54 Dipicolinic Acid, its Analogues and Derivatives: Aspects of Their Coordination Chemistry . . 161
Chapter 55 Drug Targeting by Magnetically Responsive Colloids . . 163
Chapter 56 Electrochemical Technology Applied in Treatment of Wastewater and Ground Water . . 165
Chapter 57 Electrospinning Process and Nanofiber Research . . 167
Chapter 58 Folding/Unfolding Kinetics of Lattice Proteins by Applying a Simple Statistical Mechanical Model for Protein Folding . . 169
Chapter 59 Interpolyelectrolyte Complexes of Chitosan . . 171
Chapter 60 Modern Nanochemistry . . 173
Chapter 61 Polymer Assisted Surface Modification by Photons . . 175
Chapter 62 Porous Structure and Adsorption Behaviours of Chitosan . . 177
Chapter 63 Principles of Free Radical Biomedicine. Volume I . . 179
Chapter 64 Protein-Protein Interactions . . 181
Chapter 65 Recent Developments in Bio-Nanocomposites for Biomedical Applications . . 183
Chapter 66 Sampling and Sample Preparation in Analytical Chemistry . . 185
Chapter 67 Soybeans: A Peroxidase Source for the Biotreatment of Effluents . . 187
Chapter 68 Squalene: Current Knowledge and Potential Therapeutical Uses . . 189
Chapter 69 Starch Science Progress . . 191
Chapter 70 Symbiotic Biofilms and Brain Neurochemistry . . 193
Chapter 71 Tannins and Phenolics in Animal Nutrition: Chemistry and Challenges . . 195
Chapter 72 Theoretical Estimation of Acidic Force of Linear Olefins of Cationic Polymerization . . 197
Chapter 73 Alkaloids: Properties, Application and Pharmacological Effects . . 199
Chapter 74 Intraocular Pressure Lowering Effect of Natural Ergot Alkaloids and their Future Applications in Ocular Pharmacology . . 201
Chapter 75 Pilocarpine and Related Alkaloids in Pilocarpus Vahl (Rutaceae) . . 203
Chapter 76 Quaternary Benzo[c]phenantridine Alkaloids: Perspective Fluorescence DNA probes . . 205
Chapter 77 Metabolic Engineering and Alkaloid Producing Pathways: Scopolamine as a Model Case . . 207
Chapter 78 The Effects of Alkaloids on the Feeding of Lepidopteran Larvae . . 209
Chapter 79 Biosynthesis of Chlorinated Alkaloids in Menispermum Dauricum Root Culture . . 211
Chapter 80 Lipids and Cell Function . . 213
Chapter 81 Immune Response Modulation by Targeted Complexes Based on Streptavidin . . 215
Chapter 82 Electrochemical and Optical Biosensors Based on Strept(Avidin)-Biotin Affinity . . 217
Chapter 83 Cardiac (Patho)Physiological Actions of the Classical Mu-, Delta-, and Kappa-Opioid Receptor System . . 219
Chapter 84 Angiotensin Converting Enzyme Inhibitors: A Class of Potent Antihypertensive Agents . . 221
Chapter 85 Protein Superfamilies Based Phylogenomic Analysis of Archaeal Domain . . 223
Chapter 86 Aptamers: The New Biorecognition Element for Proteomic Biosensing . . 225
Chapter 87 Effect on Heavy Oils by Bacteria . . 227
Chapter 88 Effect of Forest Environments on Human Urinary Adrenaline . . 229
Chapter 89 Microarray-Based Assay for Screening Kinase Inhibitors by Biotinylated Gold Nanoparticle Probes . . 231
Chapter 90 ATP at a Crossroads: Cell Life or Death? . . 233
Chapter 91 Bioenergetics of Closed Ecological Systems: Effects of Carbon Sources . . 235
Chapter 92 The Role of Mitochondria in the Glucose Metabolism . . 237
Chapter 93 Mitochondrial Dysfunction Produced by Zn (II) or Selenite: A Comparison with Cd (II) and Ca (II) . . 239
Chapter 94 Extramitochondrial Aerobic Metabolism in Retinal Rod Outer Segment Disks . . 241
Chapter 95 Bioenergetics and Male Infertility: From Basic Science to Clinical Andrology . . 243
Chapter 96 Identification of Specific Mitochondrial Proteins Forming Stable Adducts with 4-Hydroxynonenal within Cardiac Tissue of Type-I Diabetic Animals: Implications for Bioenergetics Dysfunction and Onset of Diabetic Cardiomyopathy . . 245
Chapter 97 Simultaneous Fluorescence and Reflection Confocal Microscopy Study of Living Osteoblast Bioenergetics as a Tool for the Design of Surface Topography of Dental Implants . . 247
Chapter 98 Screening and Studying Photosynthetic Mutants: Basics and Beyond . . 249
Chapter 99 Bioengineering Approaches for Aetiological Research in Severe Pressure Ulcers . . 251
Chapter 100 Ion Beam Bioengineering Research in Thailand . . 253
Chapter 101 Analysis of Shell Structures Applying Triangular Finite Elements . . 255
Chapter 102 Bioengineering of Human Fetal Tissues for Clinical Use . . 257
Chapter 103 Gene Cluster or Operon Design by Ordered Gene Assembly in Bacillus Subtilis (OGAB) Method . . 259
Chapter 104 Bioengineering of Glass-Ceramics and Ceramics for Dental Restoration . . 261
Chapter 105 Development of a Diagnostic System for Osteoarthritis Using a Photoacoustic Measurement Method and Time-Resolved Auto-Fluorescence . . 263
Chapter 106 Biotechnology and Agriculture . . 265
Chapter 107 Calcium Orthophosphate-Based Biocomposites and Hybrid Biomaterials . . 267
Chapter 108 Calcium Orthophosphate Cements and Concretes . . 269
Chapter 109 Hydrogels in Biology and Medicine . . 271
Chapter 110 Biomaterials in Dentistry and Medicine . . 273
Chapter 111 Biomaterials in Blood-Contacting Devices: Complications and Solutions . . 275
Chapter 112 Biocompatibility of Dental and Medical Materials . . 277
Chapter 113 Enhancing Remineralization of Subsurface Enamel Lesions with Functionalized (β-TCP) . . 279
Chapter 114 Translation of Emerging Hydrogel Therapies: The Role of Metrology . . 281
Chapter 115 Metal Ion Release from the Base Co-Cr-Mo, Ni-Cr, and Noble Au-Pt Dental Alloy into the Buffered Solutions of Different Composition and pH Value . . 283
Chapter 116 Co-Cr-Mo Alloy Surface Features and Composition Prior and after Mechanical Polishing and Corrosion in Fluids Simulating Oral Conditions . . 285
Chapter 117 Intracellular Delivery of Gold Nanoparticles: Applications in Nanomedicine . . 287
Chapter 118 A Commentary on Neural Tissue Engineering in the Central Nervous System — Interfacing a Lesion . . 289
Chapter 119 Biosurfactants and Their Uses in the Petroleum Industry and Hydrocarbon Pollution Remediation . . 291
Chapter 120 Detergents in Molecular Biology: DNA Extraction and Purification . . 293
Chapter 121 Carbohydrases in Detergents . . 295
Chapter 122 The Influence of Different Detergents on the Preservation of Discrete Membrane Microdomains: A Comparative Ultrastructural Study on Whole-Mounted Colorectal Cancer Cells . . 297
Chapter 123 -Sulfo Fatty Methyl Ester Sulfonates (Φ-MES): A New Anionic Surfactant . . 299
Chapter 124 Acacia Caven (Mol.) Molina Pollen Proteases: Application to the Peptide Synthesis and to Laundry Detergents . . 301
Chapter 125 Pharmacogenetics of Glucocorticoid Response . . 303
Chapter 126 The Glucocorticoid Receptor Signaling in Cancer and Inflammation . . 305
Chapter 127 Glucocorticoids - Age Specific Impact on Mitochondrial Energy Homeostasis . . 307
Chapter 128 Glucocorticoids, Stress and Cancer: New Insights in Immune System and Tumour Cell Regulations . . 309
Chapter 129 Hypothalamic Obesity: A Pivotal Role of Endogenous Glucocorticoid . . 311
Chapter 130 The Role of Nitric Oxide - Redox Imbalance in Glucocorticoid-Induced Hypertension . . 313
Chapter 131 Glucocorticoids Mediate Drug Resistance: Mechanisms and its Clinical Impact . . 315
Chapter 132 Glucocorticoids in Perioperative Care . . 317
Chapter 133 Glucocorticoid-Mediated Regulation of Tight Junctions in Brain Vascular Endothelium . . 319
Chapter 134 Glucocorticoid Therapy for Cochlear Injuries: Pharmacology and Evidence from Animal Experiments . . 321
Chapter 135 Expert Commentary: Glucocorticoids and Exercise Performance in Humans: Current Status . . 323
Chapter 136 Glucocorticoids, Thymus Function and Sex Hormone in Human Body Growing . . 325
Chapter 137 Nutritional Factors and Osteoporosis Prevention . . 327
Chapter 138 Regulation of Phosphate Transport in Epithelia . . 329
Chapter 139 Methods for the Extraction of Metabolites from Plant Tissues . . 331
Chapter 140 Application of 'Omics' Technologies for Improvement of Meat Quality . . 333
Chapter 141 Role of Chaperones in Dystrophic and Senescent Skeletal Muscle Fibres . . 335
Chapter 142 Amino Acid Composition of Some Aquaculture Fauna Resources in Nigeria . . 337
Chapter 143 The Biochemistry of Isoelectric Processing and Nutritional Quality of Proteins and Lipids Recovered with This Technique . . 339
Chapter 144 Nutritional Biochemistry of Curcumin (Diferuloylmethane) and a Review of Its Biological Actions on Articular Chondrocytes . . 341
Chapter 145 Effect of Creatine Applied as Food Supplement on Human Metabolism . . 343
Chapter 146 Production, Properties and Stability of Chicken Meat Protein Hydrolysate Powder . . 345
Chapter 147 Alpha-Galactosidase: A Food and Feed Enzyme . . 347
Chapter 148 Foods of Plant Origin as Source of Nitric Oxide Production Inhibitors . . 349
Chapter 149 Application of Plant-Derived Food Lectins in Proteoglycomics and Immunomodulation . . 351
Chapter 150 Biochemical and Proteomic Profiling of Key Metabolic Enzymes in Aging Skeletal Muscle . . 353
Chapter 151 Inhibitory Mechanism of Longer Chain Fatty Acids on Mammalian DNA Polymerase β Activity . . 355
Chapter 152 Endorhizal Fungi Associated with Vascular Plants on Truelove Lowland, Devon Island, Nunavut, Canadian High Arctic . . 357
Chapter 153 Involvement of the Mitochondrial ATP-Sensitive Potassium Channel in the Beneficial Effects of Fasting on the Ischemic-Reperfused Rat Heart . . 359
Chapter 154 Application of Artificial Neural Network for Predicting Physicochemical Properties of Pressure-Induced Gel from Industrial Milk Whey Proteins . . 361
Chapter 155 Isoflavones: a Race after the Rescue of the Ageing Hippocampus . . 363
Chapter 156 Prevention of Cardiovascular Diseases by Soy Protein and Isoflavones . . 365
Chapter 157 Effects of Processing on Isoflavone Content and Profile in Foodstuffs: A Review . . 367
Chapter 158 Dietary Isoflavones and Intestinal Microbiota: Metabolism and Transformation into Bioactive Compounds . . 369
Chapter 159 Bioactive Isoflavones: Implications in Cancer Treatment and Prevention . . 371
Chapter 160 Comparative in Vitro Effects of Soybean Isoflavones and Resveratrol on Human Osteoblastic Cells . . 373
Chapter 161 Health Effects of Dioscorea Bulbifera of Aerial-Potato (Yam) . . 375
Chapter 162 Effects of the Processing Techniques on Isoflavone Profiles . . 377
Chapter 163 Structural Studies and Mechanisms of Isoflavonoid Biosynthesis . . 379
Chapter 164 Photophysics of Genistein and Biochanin a Isoflavones: Solvent Cage and Concentration Effects Studied by UV Visible Spectroscopy . . 381
Chapter 165 Soy Isoflavone Genistein in Cancer Chemoprevention . . 383
Chapter 166 Isoflavones in Leguminosae . . 385
Chapter 167 Diffuse Neutron Scattering Study of Correlation Effects among Thermal Displacements of Atoms . . 387
Chapter 168 Small Angle Scattering Analysis of Virus-like Particles for Biomedical Diagnostic Assays . . 391
Chapter 169 Small Angles Neutrons Scattering Application in Biological Materials . . 391
Chapter 170 Neutron Scattering in Skin Research . . 393
Chapter 171 Precipitate Analysis of Microalloyed Steels using Small Angle Neutron Scattering . . 395
Chapter 172 Small Angle Neutron Scattering from Proteinsin Solution . . 397
Chapter 173 Neutron and X-Ray Diffraction Studies of Nanoparticles Confined within Porous Media . . 399
Chapter 174 Methods in Protein Structure and Stability Analysis: Conformational Stability, Size, Shape and Surface of Protein Molecules . . 401
Chapter 175 Neutron Scattering from Solutions of Biological Macromolecules . . 403
Chapter 176 Elastic, Quasi Elastic and Inelastic Neutron Scattering Studies on Hydrogen-Bonded Systems of Biophysical Interest . . 405
Chapter 177 Milk Oligosaccharides . .407
Chapter 178 Prebiotic Oligosaccharides: Origins and Production, Health Benefits and Commercial Applications . . 409
Chapter 179 Glycoside Hydrolases from Hyperthermophiles: Structure, Function and Exploitation in Oligosaccharide Synthesis . . 411
Chapter 180 Enzymatic Synthesis of Linear, Cyclic and Complex Type Oligosaccharides . . 413
Chapter 181 Precursor N-Linked Oligosaccharides as Codes for Glycoprotein Folding Status . . 415
Chapter 182 Oligosaccharides from Sucrose via Glycansucrases . . 417
Chapter 183 Electrospray Mass Spectrometry of Oligosaccharides of Plant Origin . . 419
Chapter 184 Biologically Active Oligosaccharide Functions in Plant Cell: Updates and Prospects . . 421
Chapter 185 The Mannan Oligosaccharides in Aquaculture . . 423
Chapter 186 Facile Synthesis of Unnatural Oligosaccharides by Phosphorylase-catalyzed Enzymatic Glycosylations Using New Glycosyl Donors . . 425
Chapter 187 Oligosaccharides: Sources, Properties and Applications . . 427
Chapter 188 Initiators for Anionic Polymerization: Old and New Developments . . 429
Chapter 189 Advanced Organocobalt Initiators of Radical Polymerization: Current State and Potential Applications . . 431
Chapter 190 Surface-Initiated ATRP as a Versatile Method to Hybrid Materials . . 433
Chapter 191 Citric Acid: An Emerging Substrate for the Formation of Biopolymers . . 435
Chapter 192 Functional Initiators in Radical Polymerization . . 437
Chapter 193 Assessing the Effect of Selected Photoinitiators on the Properties of Photopolymerised Temperature Sensitive Hydrogels . . 439
Chapter 194 Functional Oligomeric Azo-Initiators for Grafting of Polymers on Clay Surface . . 441
Chapter 195 Photoinitiating Systems Based on Unusual Radicals . . 443
Chapter 196 Role of Monocationic and Bicationic Initiators on the Grafting of Polymer Chains 'from' the Clay Surface . . 445
Chapter 197 Designing Non-Classical Inclusion Bodies . . 447
Chapter 198 Extraribosomal Functions of Ribosomal Protein SI9" in Monograph Ribosomal Proteins . . 449
Chapter 199 Order-Disorder Transitions in Ribosome Assembly . . 451
Chapter 200 Disruption of Ribosomal Protein Gene Expression in Eukaryotes: A Class of Mutations Causing Similar Global Growth Defects with Cellular Variations . . 453
Chapter 201 Ancestral Protein Reconstruction as a New Field in Protein Engineering . . 455
Chapter 202 Ribosomal Proteins in Important Tropical Diseases . . 457
Chapter 203 Three Challenges in Post-Proteomics Time . . 459
Chapter 204 Self-similar Property of Ribosomal Proteins . . 461
Chapter 205 Synthesizing Mammalian Cell Adhesive Surfaces using Self-Assembling Fibronectin Domains . . 463
Chapter 206 Halophilic Adaptations of Proteins . . 465
Chapter 207 Alkaline Adaptation of Proteins . . 467
Chapter 208 Preface to the Inaugural Issue of the Journal of Biochemistry and Molecular Biology in the Post-Genomic Era . . 469
Chapter 209 Biochemistry and Molecular Biology in Hong Kong: Past and Future . . 473
Chapter 210 Calpains-Mediated PTEN Cleavage in Excitotoxic Neuronal Death . . 477
Chapter 211 NUDCD2 Is Overexpressed in Head and Neck Squamous Cell Carcinoma and Is Involved in Nuclear Division . . 479
Chapter 212 Genotype Analyses Using SNP (Using MALDI-TOF Mass Spectrometry) and STR (Microsatellite) Markers in the Determination of Zygosity Status of Chinese Twins . . 481
Chapter 213 The Dyslexia Candidate Gene Kiaa0319L Encodes N-Glycosylated Isoforms That Form Homo-Dimers . . 483
Chapter 214 Identification of Feed Forward Loops Composed of MicroRNAs and Transcription Factors in Arabidopsis . . 485
Chapter 215 Sanfilippo Syndrome: Identification and Removal of a Cryptic Splice Site in Alpha-N-Acetylglucosaminidase cDNA for Potential Improved Expression and Enzyme Replacement Therapy . . 487
Chapter 216 Conference Report of 2010 Inter-University Post-Graduate Symposium . . 489
Chapter 217 Launching Theme-Based Research in Hong Kong . . 491
Chapter 218 Chaos Theory in Astrophysics, Nature, Genetics, and Human Cancer . . 493
Chapter 219 Structural and Functional Studies of Zonula Occludens-1 . . 495
Chapter 220 Global Gene Expression Changes during Chondrocyte Adaptation to ER Stress . . 497
Chapter 221 Genetic Analysis of Zebrafish Hematopoiesis-Defective Mutant Thy-193 . . 499
Chapter 222 Structural and Functional Analysis of Influenza H5N1 Nucleoprotein Reveals Amino Acids That Are Crucial for Oligomerization, RNA Binding and Ribonucleoprotein Activities . . 501
Chapter 223 Structural Basis of the Organization of the USH1 Interactome . . 503

Preface . . vii

Chapter 1 The Varied Functions of Aurora Kinases A and B in Mitosis and Carcinogenesis . . 1
Chapter 2 New Developments in Chromatin Research: An Epigenetic Perspective . . 29
Chapter 3 Sperm Chromatin Integrity, DNA Fragmentation and Male Fertility . . 59
Chapter 4 The Chromatin State of Pluripotent Stem Cells . . 83
Chapter 5 MITF Meets Chromatin in Melanoma . . 105
Chapter 6 The State of Chromatin as an Integrative Indicator of Cell Stress . . 123
Chapter 7 A Genomics Approach to Analysing DNA Damage and Its Repair throughout Entire Genomes . . 145
Chapter 8 Recent Patent on Revolver-2: A Novel Transposon-Like Gene Useful for Chromosome Tags of Rye . . 161
Chapter 9 The Removal of DNA Damage Is Promoted by the Yeast Global Genome Nucleotide Excision Repir Factor Rad16 . . 177
Chapter 10 Chromatin Condensation in Infertile Sperm . . 195
Chapter 11 Glucocorticoid-Induced Chromatin Remodeling: A Novel Molecular Mechanism of Traumatic Stress . . 211

Index . . 223

Preface . . vii

Chapter I Potassium Channels and Vascular Tone: Structure, Regulation and Function . . 1
Chapter II The Contribution of Lymphocyte Potassium Channels to the Perinatal Regulation of the Immune Response in Mother and Newborn . . 49
Chapter III Mitochondrial Potassium Channel: Features and Physiological Participation . . 69
Chapter IV Slow Potassium Channel Dysfunction in Amyotrophic Lateral Sclerosis and Its In Vivo Evaluation by Threshold Tracking . . 83
Chapter V Plant Mitochondrial Potassium: Channel or Channels? . . 97
Chapter VI Involvement of the Mitochondrial ATP-Sensitive Potassium Channel in the Beneficial Effects of Fasting on the Ischemic-Reperfused Rat Heart . . 119

Index . . 135

Preface to the Series . . v
Preface . . vii
Contributors . . xi

1. In Vivo Functional Brain Imaging Using a Genetically Encoded Ca2+-Sensitive Bioluminescence Reporter, GFP-Aequorin . . 1
2. Ca2+ Imaging in Brain Slices Using Bioluminescent Reporters . . 27
3. Calcium Imaging of Neural Activity in the Olfactory System of Drosophila . . 43
4. Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin . . 71
5. Performing Electrophysiology and Two-Photon Calcium Imaging in the Adult Drosophila Central Brain During Walking Behavior . . 83
6. In Vivo Optical Recording of Brain Interncuron Activities from a Drosophila Male on a Treadmill . . 103
7. Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies . . 113
8. Engineering and Application of Genetically Encoded Calcium Indicators . . 125
9. Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps . . 149

Index . . 169

Preface . . vii
Chapter I Interferon Regulatory Factors in Immune Cell Development and Host Response to Infections . . 1
Chapter II Interferon-Gamma, Oligodendrocyte Injury and Inflammatory Demyelination . . 67
Chapter III Interferons and Their Effect on NK Cells Activity in HIV Infection and Tumour Cells Development . . 83
Chapter IV Interferons, Serine Proteases and Protease-Activated Receptors: Enemies or Partners? . . 95
Chapter V The Roles of IFN- y in Esophageal Squamous Cell Carcinoma . . 107
Chapter VI Interferons in Multiple Sclerosis: Clinical Applications and Possible Mechanisms of Actions . . 119
Chapter VII Dynamics of Lymphocyte Subpopulations in Patients with Chronic Hepatitis C Virus Infection During Pegylated Interferon-Alpha2a and Ribavirin Treatment . . 129
Chapter VIII Co-Administration of Gamma Interferon Gene with DNA Vaccine Expressing Duck Hepatitis B Virus (DHBV) Proteins Enhances Therapeutic Efficacy of DNA-Based Immunization in Chronic Virus Carriers . . 135
Chapter IX Rabies Virus Evasion of the Interferon System . . 163
Index . . 163